An Introduction to BioBricks

BioBricks is a standard for interchangable parts, developed with a view to building biological systems in living cells. The picture to the left shows BioBrick part BBa_B0034 within a plasmid. The brick is flanked by a standard BioBrick prefix (P) and suffix (S). Click the image to find out more or alternatively see: BioBrick Names Types of BioBrick Plasmids Introduction BioBrick Prefix and Suffix

Building BioBrick Systems

BioBrick parts can be assembled to form useful devices, through a process often referred to as 'Standard Assembly'. This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. BioBrick parts are composable; allowing endless numbers of biobricks to be pieced together to form complex systems. The assembly to the left shows 3 BioBricks assembled to create a simple device. Standard assembly BioBrick restriction enzymes Rolling assembly Automated assembly - under development

Designing BioBrick Devices & Systems: Tools & Methods

The Registry provides several tools that will aid you in designing and understanding biobrick systems and devices. Adding a part to the Registry allows you to quickly and easily manipulate new designs. The search facility allows you to quickly and easily scour the Registry for information, trace the origin of more complex parts and see which higher systems have been constructed with any part of choice. Having designed your biobricks using the Registry tool set, you'll want to physically make your BioBrick; the online help system gives detailed guides to protocols and procedures relating to this topic, see: Techniques How to make a BioBrick [http://openwetware.org/wiki/BioBricks_construction_tutorial How to perform a construction] Standardization of non-Biobrick sequence Tools Add a part to the Registry Part search facility Sequence Analysis Tool Assembly Tool - under development