Coding
eforRed

Part:BBa_K592012

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 00:42, 26 September 2013 by Earthnut (Talk | contribs)

eforRed, red chromoprotein

This chromoprotein eforRed naturally exhibits red/pink color when expressed. The color is slightly weaker than RFP. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.

This part can also be found as a coding device BBa_K864402.

Usage and Biology

This part is useful as a reporter.

Eforred plate small.jpg UUChromo.jpg iGEM12_Uppsala_University: Left picture: Visual color expression of eforRed (BBa_K592012).
Right picture: The Uppsala chromoprotein collection and RFP. The image shows pellets of E coli expressing chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.

Getting this part

All chromoproteins displayed above should be available, but if the registry cannot send them, feel free to contact the Uppsala University team directly via erik.gullberg(at)imbim.uu.se, and we will send them to you.

References

[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


<style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum { text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%; } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style>
Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K592012">BBa_K592012</a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)
 ATGGCCGGCATGTCAGTG ... GCTCTCCCAACCGGT
 ORF from nucleotide position -8 to 687 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

101 
201 
MAGMSVIKQVMKTKLHLEGTVNGHDFTIEGKGEGKPYEGLQHMKMTVTKGAPLPFSVHILTPSHMYGSKPFNKYPADIPDYHKQSFPEGMSWERSMIFED
GGVCTASNHSSINLQENCFIYDVKFHGVNLPPDGPVMQKTIAGWEPSVETLYVRDGMLKSDTAMVFKLKGGGHHRVDFKTTYKAKKPVKLPEFHFVEHRL
ELTKHDKDFTTWDQQEAAEGHFSPLPKALPTG*
Sequence features: (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)
None of the supported features appeared in the sequence
Amino acid composition:
Ala (A)10 (4.3%)
Arg (R)4 (1.7%)
Asn (N)6 (2.6%)
Asp (D)12 (5.2%)
Cys (C)2 (0.9%)
Gln (Q)7 (3.0%)
Glu (E)15 (6.5%)
Gly (G)21 (9.1%)
His (H)14 (6.0%)
Ile (I)8 (3.4%)
Leu (L)15 (6.5%)
Lys (K)23 (9.9%)
Met (M)11 (4.7%)
Phe (F)13 (5.6%)
Pro (P)17 (7.3%)
Ser (S)13 (5.6%)
Thr (T)16 (6.9%)
Trp (W)3 (1.3%)
Tyr (Y)7 (3.0%)
Val (V)15 (6.5%)
Amino acid counting
Total number:232
Positively charged (Arg+Lys):27 (11.6%)
Negatively charged (Asp+Glu):27 (11.6%)
Aromatic (Phe+His+Try+Tyr):37 (15.9%)
Biochemical parameters
Atomic composition:C1175H1797N311O336S13
Molecular mass [Da]:26072.9
Theoretical pI:7.26
Extinction coefficient at 280 nm [M-1 cm-1]:26930 / 27055 (all Cys red/ox)
Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges <input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot()' value='Show'>
Codon usage
Organism:E. coliB. subtilisS. cerevisiaeA. thalianaP. patensMammals
Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):good (0.79)good (0.76)good (0.66)good (0.73)good (0.79)good (0.71)
Alignments (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)
   There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.
Predictions (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)
   There were no predictions for this protein in the data base. The prediction was initialized and should be ready in a few hours.
The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.
If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.

<script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>var hydrophobicity_datapoints = [[2.5,0.88],[3.5,1.34],[4.5,1.88],[5.5,1.18],[6.5,0.10],[7.5,1.10],[8.5,0.64],[9.5,-1.04],[10.5,-0.40],[11.5,-0.48],[12.5,-0.56],[13.5,-1.58],[14.5,-0.04],[15.5,-0.60],[16.5,0.10],[17.5,-0.80],[18.5,0.68],[19.5,-0.78],[20.5,-0.16],[21.5,-0.72],[22.5,-1.28],[23.5,-1.56],[24.5,-1.00],[25.5,-0.02],[26.5,-0.08],[27.5,0.54],[28.5,-0.80],[29.5,-0.74],[30.5,-2.34],[31.5,-1.72],[32.5,-2.42],[33.5,-1.96],[34.5,-2.14],[35.5,-2.14],[36.5,-2.14],[37.5,-0.60],[38.5,-0.98],[39.5,-1.36],[40.5,-0.28],[41.5,-0.98],[42.5,-1.36],[43.5,-0.80],[44.5,0.68],[45.5,0.16],[46.5,0.16],[47.5,-0.30],[48.5,0.20],[49.5,-0.96],[50.5,-0.06],[51.5,0.40],[52.5,1.04],[53.5,0.52],[54.5,1.68],[55.5,0.28],[56.5,1.50],[57.5,1.70],[58.5,1.72],[59.5,0.56],[60.5,1.04],[61.5,-0.50],[62.5,-0.88],[63.5,-1.00],[64.5,-0.76],[65.5,-0.76],[66.5,-0.90],[67.5,-1.60],[68.5,-0.78],[69.5,-1.40],[70.5,-2.02],[71.5,-1.50],[72.5,-1.50],[73.5,-1.70],[74.5,-1.70],[75.5,-0.02],[76.5,-0.08],[77.5,-0.46],[78.5,-1.08],[79.5,-1.02],[80.5,-2.70],[81.5,-3.08],[82.5,-2.54],[83.5,-1.72],[84.5,-1.40],[85.5,-1.32],[86.5,-0.70],[87.5,-0.16],[88.5,-0.88],[89.5,-0.74],[90.5,-0.74],[91.5,-1.56],[92.5,-2.10],[93.5,-1.56],[94.5,-0.48],[95.5,0.78],[96.5,0.98],[97.5,0.44],[98.5,-0.02],[99.5,-1.00],[100.5,-0.72],[101.5,0.48],[102.5,1.04],[103.5,1.48],[104.5,1.40],[105.5,-0.14],[106.5,-1.28],[107.5,-1.30],[108.5,-1.82],[109.5,-0.76],[110.5,-0.76],[111.5,0.64],[112.5,0.10],[113.5,-0.44],[114.5,-2.04],[115.5,-0.84],[116.5,-1.04],[117.5,0.56],[118.5,1.00],[119.5,1.00],[120.5,1.34],[121.5,0.00],[122.5,-0.34],[123.5,-0.72],[124.5,-0.10],[125.5,-0.10],[126.5,-0.02],[127.5,0.18],[128.5,0.50],[129.5,0.26],[130.5,-1.28],[131.5,-0.66],[132.5,-1.74],[133.5,-0.58],[134.5,0.12],[135.5,0.12],[136.5,-0.58],[137.5,-0.40],[138.5,-0.34],[139.5,-0.36],[140.5,0.26],[141.5,0.86],[142.5,0.30],[143.5,-0.92],[144.5,-1.44],[145.5,-0.52],[146.5,-1.04],[147.5,-0.48],[148.5,0.60],[149.5,0.50],[150.5,0.50],[151.5,0.30],[152.5,-0.26],[153.5,-1.10],[154.5,-0.46],[155.5,-0.54],[156.5,-0.42],[157.5,0.12],[158.5,-0.50],[159.5,-1.02],[160.5,-1.42],[161.5,-0.26],[162.5,0.74],[163.5,2.00],[164.5,1.36],[165.5,1.76],[166.5,0.60],[167.5,-0.32],[168.5,-0.96],[169.5,-0.26],[170.5,-1.66],[171.5,-1.52],[172.5,-2.34],[173.5,-1.42],[174.5,-2.04],[175.5,-0.84],[176.5,-0.98],[177.5,-0.22],[178.5,-1.20],[179.5,-0.76],[180.5,-2.10],[181.5,-0.96],[182.5,-1.60],[183.5,-2.24],[184.5,-2.30],[185.5,-0.68],[186.5,-1.82],[187.5,-0.28],[188.5,0.18],[189.5,-0.20],[190.5,-0.48],[191.5,-0.34],[192.5,-0.54],[193.5,0.62],[194.5,0.62],[195.5,-0.58],[196.5,-0.84],[197.5,-0.64],[198.5,-2.18],[199.5,-0.72],[200.5,-0.22],[201.5,-0.10],[202.5,-1.50],[203.5,-1.50],[204.5,-3.04],[205.5,-3.60],[206.5,-2.26],[207.5,-1.76],[208.5,-1.20],[209.5,-0.60],[210.5,-0.60],[211.5,-1.86],[212.5,-2.42],[213.5,-2.98],[214.5,-2.44],[215.5,-1.38],[216.5,-1.38],[217.5,-0.76],[218.5,-0.70],[219.5,-0.50],[220.5,-1.02],[221.5,-0.64],[222.5,0.20],[223.5,0.52],[224.5,-0.82],[225.5,-0.30],[226.5,0.78],[227.5,-0.30],[228.5,-0.12],[229.5,0.58]];var charge_datapoints = [[2.5,0.00],[3.5,0.00],[4.5,0.00],[5.5,0.20],[6.5,0.20],[7.5,0.20],[8.5,0.20],[9.5,0.40],[10.5,0.20],[11.5,0.40],[12.5,0.40],[13.5,0.50],[14.5,0.30],[15.5,0.10],[16.5,-0.10],[17.5,-0.10],[18.5,-0.20],[19.5,-0.20],[20.5,-0.00],[21.5,0.10],[22.5,-0.10],[23.5,-0.10],[24.5,-0.10],[25.5,-0.10],[26.5,-0.40],[27.5,-0.20],[28.5,0.00],[29.5,0.00],[30.5,-0.20],[31.5,0.00],[32.5,0.20],[33.5,0.00],[34.5,0.00],[35.5,0.00],[36.5,0.00],[37.5,-0.20],[38.5,-0.20],[39.5,-0.10],[40.5,0.10],[41.5,0.30],[42.5,0.30],[43.5,0.30],[44.5,0.20],[45.5,0.20],[46.5,0.20],[47.5,0.20],[48.5,0.20],[49.5,0.20],[50.5,0.20],[51.5,0.00],[52.5,0.00],[53.5,0.00],[54.5,0.00],[55.5,0.10],[56.5,0.10],[57.5,0.10],[58.5,0.10],[59.5,0.10],[60.5,0.00],[61.5,0.10],[62.5,0.10],[63.5,0.10],[64.5,0.10],[65.5,0.10],[66.5,0.20],[67.5,0.20],[68.5,0.20],[69.5,0.20],[70.5,0.40],[71.5,0.20],[72.5,0.20],[73.5,0.20],[74.5,0.00],[75.5,-0.20],[76.5,-0.20],[77.5,-0.40],[78.5,-0.40],[79.5,-0.10],[80.5,0.10],[81.5,0.10],[82.5,0.30],[83.5,0.30],[84.5,0.20],[85.5,-0.20],[86.5,-0.20],[87.5,-0.20],[88.5,-0.20],[89.5,-0.20],[90.5,-0.20],[91.5,0.00],[92.5,0.00],[93.5,0.00],[94.5,0.00],[95.5,0.20],[96.5,-0.20],[97.5,-0.40],[98.5,-0.40],[99.5,-0.40],[100.5,-0.40],[101.5,-0.20],[102.5,0.00],[103.5,0.00],[104.5,0.00],[105.5,0.00],[106.5,0.10],[107.5,0.10],[108.5,0.10],[109.5,0.10],[110.5,0.10],[111.5,0.00],[112.5,0.00],[113.5,-0.20],[114.5,-0.20],[115.5,-0.20],[116.5,-0.20],[117.5,-0.20],[118.5,0.00],[119.5,-0.20],[120.5,-0.20],[121.5,0.00],[122.5,0.00],[123.5,0.10],[124.5,0.30],[125.5,0.30],[126.5,0.10],[127.5,0.10],[128.5,0.00],[129.5,0.00],[130.5,-0.20],[131.5,-0.20],[132.5,-0.20],[133.5,-0.20],[134.5,-0.20],[135.5,0.00],[136.5,0.20],[137.5,0.20],[138.5,0.20],[139.5,0.20],[140.5,0.20],[141.5,0.00],[142.5,-0.20],[143.5,-0.20],[144.5,-0.20],[145.5,-0.20],[146.5,-0.40],[147.5,-0.20],[148.5,-0.20],[149.5,-0.20],[150.5,-0.20],[151.5,0.20],[152.5,0.00],[153.5,0.00],[154.5,0.00],[155.5,0.00],[156.5,0.00],[157.5,0.20],[158.5,0.00],[159.5,0.00],[160.5,0.00],[161.5,-0.20],[162.5,-0.20],[163.5,0.00],[164.5,0.20],[165.5,0.20],[166.5,0.40],[167.5,0.40],[168.5,0.40],[169.5,0.20],[170.5,0.30],[171.5,0.20],[172.5,0.40],[173.5,0.40],[174.5,0.20],[175.5,0.10],[176.5,0.20],[177.5,0.00],[178.5,0.00],[179.5,0.20],[180.5,0.40],[181.5,0.20],[182.5,0.40],[183.5,0.60],[184.5,0.60],[185.5,0.40],[186.5,0.60],[187.5,0.40],[188.5,0.20],[189.5,0.00],[190.5,0.00],[191.5,-0.10],[192.5,-0.10],[193.5,-0.10],[194.5,-0.10],[195.5,0.00],[196.5,0.10],[197.5,0.10],[198.5,-0.10],[199.5,0.10],[200.5,0.00],[201.5,0.00],[202.5,0.10],[203.5,0.10],[204.5,0.30],[205.5,0.10],[206.5,-0.10],[207.5,-0.20],[208.5,0.00],[209.5,-0.20],[210.5,-0.20],[211.5,-0.20],[212.5,-0.20],[213.5,-0.40],[214.5,-0.40],[215.5,-0.20],[216.5,-0.40],[217.5,-0.40],[218.5,-0.10],[219.5,-0.10],[220.5,-0.10],[221.5,0.10],[222.5,0.10],[223.5,0.00],[224.5,0.20],[225.5,0.20],[226.5,0.20],[227.5,0.20],[228.5,0.20],[229.5,0.00]];var dis_datapoints = undefined;var trans_datapoints = undefined;var sec_helix_datapoints = undefined;var sec_strand_datapoints = undefined;var acc_exposed_datapoints = undefined;var acc_buried_datapoints = undefined;var flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-
phobic  '], [-4.5,'hydro-
philic  ']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ], [1,'positive
 charge'], [-1,'negative
 charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};var number_of_plots = 2;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = $.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 + 0.5, (plot_num*200 + 1).toString()], [plot_num*200 + 24.5, (plot_num*200 + 25).toString()], [plot_num*200 + 49.5, (plot_num*200 + 50).toString()], [plot_num*200 + 74.5, (plot_num*200 + 75).toString()], [plot_num*200 + 99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};function show_or_hide_plot(){try {if( $('#hydrophobicity_charge_button').val() =='Show' ){$('#hydrophobicity_charge_container').css('display','block');$('#hydrophobicity_charge_button').val('Hide');var description_html = '
';description_html = description_html + '
 <input type=\'checkbox\' id=\'hydrophobicity_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for hydrophobicity (<img src=\'https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png\' alt=\'blue graph\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'charge_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for charge (<img src=\'https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png\' alt=\'red graph\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'dis_checkbox\' checked=\'checked\'> Predicted disulfid bridges (<img src=\'https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png\' alt=\'yellow circle\' height=\'10\'></img>) with the number of the bridge in the center';description_html = description_html + '
 <input type=\'checkbox\' id=\'trans_checkbox\' checked=\'checked\'> Predicted transmembrane helices (<img src=\'https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png\' alt=\'turquois bars\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'sec_checkbox\' checked=\'checked\'> Predicted secondary structure: Helices (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png\' alt=\'violet bars\' height=\'10\'></img>) and beta-strands (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png\' alt=\'yellow bars\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'acc_checkbox\' checked=\'checked\'> Predicted solvent accessability: Exposed (<img src=\'https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png\' alt=\'blue bars\' height=\'10\'></img>) and buried (<img src=\'https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png\' alt=\'green bars\' height=\'10\'></img>) residues';description_html = description_html + '
';$('#hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors();$('#AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors);}else{$('#hydrophobicity_charge_container').css('display','none');$('#hydrophobicity_charge_button').val('Show');$('#hydrophobicity_charge_explanation').html(
);}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors(){try{var plot_datasets = [[],[]];if($('#hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if($('#charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){$.plot('#hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = $('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if($('#dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('
' + (j+1) + '
');$('#hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('
' + (j+1) + '
');}}if($('#trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('
');}}if($('#sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('
');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('
');}}if($('#acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('
');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('
');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html>
[edit]
Categories
//cds/reporter/chromoprotein
//collections/chromoprotein/uppsala
//function/reporter/pigment
Parameters
None