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Part:BBa_K1088016

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-28)
Revision as of 18:20, 25 September 2013 by P.R.A (Talk | contribs)

HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible)

This biobrick expresses the Hevea brasiliensis rubber prenyltransferase when induced with arabinose under glucose scarce conditions. Arabinose binds the ara promoter regulator, AraC, and AraC bound arabinose activates the transcription of the ara promoter

Using Northern blot technique it was proved that the construct in E. coli K-12 MG1655 doesn't express the prenyltransferase when grown without arabinose. Upon addition of arabinose to the media prenyltranferase mRNA was detected. See experince for more details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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