Regulatory

Part:BBa_J23117:Experience

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-17)
Revision as of 14:40, 25 September 2013 by Gundl (Talk | contribs) (→‎Evaluation of Anderson promoter J23117 in E. coli by iGEM Göttingen 2013)

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Applications of BBa_J23117

Evaluation of Anderson promoter J23117 in B. subtilis by iGEM-Team LMU-Munich 2012

This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823013 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.

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UNIQ09333d1d7234983d-partinfo-00000000-QINU UNIQ09333d1d7234983d-partinfo-00000001-QINU

Evaluation of Anderson promoter J23117 in E. coli by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]



Shown here:
Upper two pictures: Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown.
Middle two pictures: RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown.
Bottom three pictures: qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117 .

Promotor 1:BBa_J23117
Promoter 2:BBa_J23116
Promoter 3:BBa_J23110
Promoter 4:BBa_J23118


The promoter strength was measured by using the reporter gene rfp.
Three different approaches were used: 1. RFP measurement, 2. qRT_PCR analyses and 3. single cell microscopy.
Our results from these approaches showed that BBa_J23177 has the lowest promoter activity compared to BBa_J23116, BBa_J23110 and BBa_J23118.


Fig. 1. RFP and qRT-PCR promoter analyses.


Fig. 2. Microscopic promoter analyses on single cell level
P1: BBa_J23117, P2: BBa_J23116, P3: BBa_J23110, P4: BBa_J23118, P8: control