Generator

Part:BBa_K1117009

Designed by: Chun Li   Group: iGEM13_BIT-China   (2013-09-16)
Revision as of 14:30, 25 September 2013 by JIAhaiyang (Talk | contribs)

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J23119-K115001

A strong constitutive promoter J23119 linked to the 42℃ RNA thermometer K115001.

We tested the RNA thermometer K115001 which control gene to initiate translation at 42°C, but we found that the SD region is occluded at 30°C, partial melting occurs at 37 °C, whereas an increase to 42 °C facilitates the mRNA–ribosome interaction owing to full liberation of the SD and AUG start codon. BIT-China-k1117009.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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