Regulatory

Part:BBa_J23117:Experience

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-17)
Revision as of 14:13, 25 September 2013 by Gundl (Talk | contribs) (Evaluation of Anderson promoter J23117 in E. coli by iGEM Göttingen 2013)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J23117

Evaluation of Anderson promoter J23117 in B. subtilis by iGEM-Team LMU-Munich 2012

This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823013 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.

User Reviews

UNIQ56ca4879f2d7f623-partinfo-00000000-QINU UNIQ56ca4879f2d7f623-partinfo-00000001-QINU

Evaluation of Anderson promoter J23117 in E. coli by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]



Shown here:
Upper two pictures: Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown.
Middle two pictures: RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown.
Bottom three pictures: qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117 .

Promotor 1:BBa_J23117
Promoter 2:BBa_J23116
Promoter 3:BBa_J23110
Promoter 4:BBa_J23118

J23117.jpg
PromoterGoe.jpg