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Composite

Part:BBa_K1045016

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 13:49, 23 September 2013 by Nina Heckmann (Talk | contribs)

Terminator.png

n/aTerminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix

Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix

  • This composite part is composed of part BBa_K1045001, an inverse DarR coding sequence, fused to an inverse terminator, BBa_K1045009. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In E. coli, DarR seemed to act as a quite strong repressor even without c-di-AMP. Part BBa_K1045016 could be cloned downstream of inverse promoter-RBS constructs (compare parts BBa_K1045010 (inverse RBS) and BBa_K1045011 (inverse promoter)) allowing it to be expressed (for example, see part BBa_K1045017).

Secondary Structure

File:Mfold-K1045016-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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n/aTerminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix