An Introduction to BioBricks

BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part BBa_0034 within a plasmid. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. For more information see: BioBrick Names Types of BioBrick Plasmids Introduction

Building BioBrick Systems

BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. BioBrick parts are composable; allowing endless numbers of biobricks to be pieced together to form higher systems. The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into: Standard Assembly - The classical method of BioBrick assembly, done at the lab bench BioBrick Restriction Enzymes Rolling Assembly Automated assembly - currently in late stage development at iGEM labs

How do I make a BioBrick?

At the bench

Pic of biobrick flanked by coding seq

Methods:

• Synthesis
• PCR

More information on putting sequence into standardized Biobrick form can be found here

Adding the part to the registry

This protocol is quick and easy. A comprehensive guide can be found here