Composite
pCI-mCherr
Part:BBa_K1157013
Designed by: Lin Ang Chieh Group: iGEM13_NTU-Taida (2013-09-15)
Revision as of 16:01, 22 September 2013 by Darlisereno (Talk | contribs)
pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI
This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 944
Illegal NheI site found at 967 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1241
Illegal BamHI site found at 2197 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1716
Illegal BsaI site found at 2672
[edit]
Categories
Parameters
//function/cellsignalling
None |