Composite

Part:BBa_K1073035

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 13:45, 17 September 2013 by Registry (Talk | contribs)

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Inducible ampR expression combined with RhlR, LasI and eforRed expression cassettes

This construct is the basis for a Quorum sensing system for two bacteria. The selection marker (amp-resistance) is induced by a RhlI(inducer)/RhlR(activator) complex. The RhlR is encoded on the construct itself, whereas the RhlI is taken up from the media. Furthermore the LasI synthetase is expressed to synthesize the autoinducer LasI for the corresponding system in another cell (BBa_K1073034). By the activation of the amp-resistance the gene of interest (in this case the chromoprotein eforRed) is expressed. Together with BBa_K1073034 the construct forms a dependency between two cells (one with BBa_K10733034, the other with BBa_K1073035).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
    Illegal NheI site found at 1119
    Illegal NheI site found at 2784
    Illegal NheI site found at 2807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2626
    Illegal BamHI site found at 1391
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2188
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1866


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Categories
Parameters
None