Composite

Part:BBa_K1073031

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 13:15, 17 September 2013 by Registry (Talk | contribs)

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Autoinducer synthase LuxI + LVA with combined amilGFP chromoprotein expression cassette

This part is the basis for the expression of the Lux auto inducer synthetase, which synthesizes LuxI [N-(3-oxohexanoyl)-homoserine lactone]. LuxI binds to the activator LuxR. Together they activate the Lux promoter. A constitutive expression of amil GFP is added to the construct in order to varify the expression of LuxI synthetase. The construct has no promoter in front of the LuxI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 814
    Illegal NheI site found at 837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 656
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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