Composite

Part:BBa_K1149005

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-14)
Revision as of 18:29, 15 September 2013 by Margarita K (Talk | contribs)

pelB-pudA expression

This part can express a plastic degradation enzyme from Comamonas acidovorans that breaks down ester bonds in polyurethane.


Theoretical pathway of PUR degradation by esterase: PUR_esterase_theoretical_pathway.jpg


references:

[http://www.selu.edu/acad_research/depts/biol/faculty/publications/pdf/2012/howard2012.pdf Howard, Gary 2012 pg:371 -3, Polyurethane Biodegradation, Environmental Science]

[http://www.sciencedirect.com/science/article/pii/S0922338X99890011 Cloning and sequence analysis of a polyurethane esterase of Comamonas acidovorans 1998]

[http://www.sciencedirect.com/science/article/pii/S0964830598000663Purification and characterization of a soluble polyurethane degrading enzyme from Comamonas acidovorans 1999 ]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 529
    Illegal AgeI site found at 604
    Illegal AgeI site found at 1753
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None