Composite

Part:BBa_K1123003

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-08)
Revision as of 13:30, 8 September 2013 by Registry (Talk | contribs)

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1PJN Protein Production Construct

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the 1PJN Protein sequence. The FNR promotor sequence enables us to bring the 1PJN protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The 1PJN protein is a protein which we are expressing with the sole purpose of using it as a CEST contrast agent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal XhoI site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None