Coding
Part:BBa_K1159003
Designed by: TU Munich 2013 Group: iGEM13_TU-Munich (2013-05-17)
Revision as of 20:45, 26 July 2013 by ChristopherW (Talk | contribs)
Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]
| Automatically determined parameters using the BioBrick-AutoAnnotator | |||||||||||||||||||
| Nucleotide sequence: (underlined part of entry was translated, italic parts were added) GTACACAATGCGTCGT...TTCGAAAAATAA | |||||||||||||||||||
| Amino acid sequence: MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK | |||||||||||||||||||
| BioBrick: | Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added). | ||||||||||||||||||
| A (Ala) | 33 (5.83%) | R (Arg) | 25 (4.42%) | N (Asn) | 27 (4.77%) | D (Asp) | 34 (6.01%) | C (Cys) | 4 (0.71%) | ||||||||||
| Q (Gln) | 24 (4.24%) | E (Glu) | 48 (8.48%) | G (Gly) | 29 (5.12%) | H (His) | 18 (3.18%) | I (Ile) | 39 (6.89%) | ||||||||||
| L (Leu) | 64 (11.31%) | K (Lys) | 36 (6.36%) | M (Met) | 16 (2.83%) | F (Phe) | 28 (4.95%) | P (Pro) | 17 (3.00%) | ||||||||||
| S (Ser) | 33 (5.83%) | T (Thr) | 26 (4.59%) | W (Trp) | 14 (2.47%) | Y (Tyr) | 27 (4.77%) | V (Val) | 24 (4.24%) | ||||||||||
| Amino acid counting: | Total number of amino acids (aa): | 566 | Number of positively charged aa (Arg + Lys): | 61 | Number of negatively charged aa (Asp + Glu): | 82 | |||||||||||||
| Biochemical parameters: | Molecular weight [Da]: | 66105.33 | Theoretical pI: | 5.38 | Extinction coefficient: [M^-1 cm^-1] | 117480 (all Cys as cystine), 117230 (no Cys as cystine) | |||||||||||||
| Estimated half-life: | Mammals: | 30 hour | Yeast: | >20 hour | E. coli: | >10 hour | |||||||||||||
| Codon usage: (CAI) | Mammals: | 0.68 | Yeast: | 0.69 | E. coli: | 0.71 | |||||||||||||
| RFC standard: | This is a RFC 10 BioBrick, nothing was added | ||||||||||||||||||
| The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description. | |||||||||||||||||||
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/ppatens
| None |