Coding

Part:BBa_K1104999:Design

Designed by: Ting-Yun Chiang   Group: iGEM13_NYMU-Taipei   (2013-06-10)
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Design Notes

We insert this coding to make E.coli produce Chitinase-63, in order to damage the spore wall of our target --pathogenic fungi, and kill them.


Source

Phillips W. Robbins, Charles Albright, and Barbara Benfield(1988),Cloning and Expression of a Streptomyces plicatus Chitinase (Chitinase-63) in Escherichia coli. Vol. 263, No. 1, Issue of January 5, 443-447. REFERENCE 1 (sites) Robbins,P.W., Overbye,K., Albright,C., Benfield,B. and Pero,J. (1992)Cloning and high-level expression of chitinase-encoding gene of Streptomyces plicatus. Gene 111 (1), 69-76. REFERENCE 2 (bases 1 to 2276) Delic,I., Robbins,P. and Westpheling,J. (1992) Direct repeat sequences are implicated in the regulation of two Streptomyces chitinase promoters that are subject to carbon catabolite control. Proc. Natl. Acad. Sci. U.S.A. 89 (5), 1885-1889.

References