Test2

Revision as of 23:24, 6 March 2013 by VolkerMorath (Talk | contribs) (Luciferases)

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Biosafety: Parts improving biological safety, containment.
Biosynthesis: Parts involved in the production of chemicals and metabolites are listed here.
Biodegradation: Parts involved in the degradation of chemicals are listed here.
Cell-cell signaling and quorum sensing: Parts involved in intercellular signaling and quorum sensing between bacteria.
Cell death: Parts involved in killing cells.
Coliroid: Parts involved in taking a bacterial photograph.
Conjugation: Parts involved in DNA conjugation between bacteria.
DNA binding: Parts involved in xxx.
DNA recombination: Parts involved in DNA recombination are listet here.
Ions: Parts involved in sensing, transport and binding of different ions are listet in here.
Ligand Binding: Parts involved in binding chemical structures are listet here.
Light production and sensing: Parts involved in generation and sensing of light are listet here.
Motility and chemotaxis: Parts involved in motility or chemotaxis of cells are listet here.
Odor production and sensing: Parts that produce or sense odorants aer listet here.
Sensors: Parts used as biosensors are listed here.
Viral vectors: Parts involved in the production and modification of Viral vectors are listet here..












Fluorescent Proteins

Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon], [http://2012.igem.org/Team:Uppsala_University/Chromoproteins Chromoproteins Uppsala] https://parts.igem.org/Part:BBa_K180009 mBanana

Name Exitation [nm] Emmission [nm] Quant. yield Molar extinct. BioBrick RFC10 BioBrick RFC25 Reference
Cerulean 433 475 0.62 43000 BBa_E2020
GFPmut3b 501 511 BBa_E0040
amilGFP 503 512 0.67 75200 BBa_K592010 [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
mVenus 515 528 0.57 92200 BBa_I757008
mOrange 548 562 0.69 71000 BBa_E2050
mStrawbery 574 596 0.29 90000 BBa_K399001
mRFP1 584 607 0.25 50000 BBa_E1010
mCherry 587 610 0.22 72000 BBa_E2060
eforRed 589 609 0.16 111300 BBa_K592012 [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
cjBlue 610 BBa_K592011 [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
aeBlue BBa_K864401 [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
amilCP 588 NA NA 8760 BBa_K592009 [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]



More...
NameTypeDescriptionLengthStatus
BBa_K125502CodingphaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993)726In stock
BBa_K759005CodingphaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)738In stock
BBa_K759007Translational_UnitRBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)756In stock
BBa_K125501CodingphaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)1233It's complicated
BBa_K125503CodingphaC BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1830) 1140It's complicated
BBa_K125504CodingphaE BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1829) 996It's complicated
BBa_K125801Translational_UnitRBS-phaA1251It's complicated
BBa_K125803Translational_UnitRBS-phaC1158It's complicated
BBa_K125804Translational_UnitRBS-phaE1014It's complicated
BBa_K156012CodingphaA (acetyl-CoA acetyltransferase)1182It's complicated
BBa_K156013CodingphaB1 (acetyacetyl-CoA reductase)741It's complicated
BBa_K156014CodingphaC1 (Poly(3-hydroxybutyrate) polymerase)1349It's complicated
BBa_K156015Translational_UnitRBS + phaA1201It's complicated
BBa_K156016Translational_UnitRBS + phaB1760It's complicated
BBa_K156021GeneratorPromoter + RBS + phaA + double terminator1400It's complicated
BBa_K156022GeneratorPromoter + RBS + phaB1 + double terminator959It's complicated
BBa_K156031GeneratorRBS + phaA + double terminator1338It's complicated
BBa_K156033GeneratorRBS + phaB1 + double terminator897It's complicated
BBa_K338003CompositePHA Synthase Composite, Part 1/21303It's complicated
BBa_K338004CompositePHA Synthase Composite, Part 2/22253It's complicated
BBa_K338023CompositePlastic Intermediate: phaB1 + RBS761It's complicated
BBa_K338022CompositePlastic Intermediate: phaA + RBS1202It's complicated
BBa_K338024CompositePlastic Intermediate: phaC1 + Terminator1486It's complicated
BBa_K759004CodingphaA (beta-ketothiolase orgin from Ralstonia eutropha)1182It's complicated
BBa_K759006Translational_UnitB0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)1200It's complicated
BBa_K759008Translational_UnitB0034 and phaC (BBa_K342001)1809It's complicated
BBa_K759009Translational_UnitRBS-phaC-RBS-phaA-RBS-phaB3781It's complicated
BBa_K759010CompositeP3HB producing construct (without a promoter)3918It's complicated
BBa_K759011CompositeP3HB producing construct 3980It's complicated
BBa_K2783010CodingPhenylalanine ammonia lyase 22154It's complicated
BBa_K125802Translational_UnitRBS-phaB744Not in stock
BBa_K156018CompositePromoter + RBS + phaA1263Not in stock

Luciferases

Name Organism Substrate pH optimum Amino acids Mol. mass [kDa] Emmission [nm] BioBrick RFC10 BioBrick RFC25 Reference
Photinus/Firefly luciferase D-luciferin + ATP 550 60.6 578 BBa_I712019
Renilla luciferase coelenterazine + O2 311 36.0 480 BBa_J52008
Gaussia luciferase coelenterazine + O2 189 20.3 480 BBa_K509005
Nano luciferase furimazine 171 19.1 460 [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]


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Photoproteins

(Aequorin, Obelin