Help:Plasmid backbones/Features

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  • Information on what the feature tables of plasmids mean

Copy Number

Most Biobricks are "high copy" type plasmids and are well-suited for high yield plasmid purification. These plasmids can reach large numbers by being replicated using host enzymes (source: [http://www.biochem.wisc.edu/courses/biochem651_f05/Lectures/08Plasmids/Theory_plasmids/copynumber.html University of Wisc. Madison])

Large DNA inserts can greatly reduce the copy number of a plasmid, despite the fact that they may originally be high-copy.

Origin (of replication)

The origin of replication is a binding sequence where replication proteins which nick, unwind, and replicate plasmid DNA independently of the cellular host. Many biobrick plasmids have the following types of origins of replication:

  • pmB1 - This origin has been isolated from one of the first plasmids taken from a human body. It is a "relaxed" plasmid, meaning that it allows high copy amounts within a cell
  • psC101 -

This origin is not to be confused with the "origin of transfer", the site used in conjugal rolling circle replication transfer.

Links and References

  • [http://dwb.unl.edu/Teacher/NSF/C08/C08Links/mbclserver.rutgers.edu/~sofer/cloningvectors.html "Cloning Vectors and Genetic Engineering"]