Coding
SoDA

Part:BBa_M36071:Design

Designed by: Alex Rosay, Justin Diep, Ben Blankenmeister   Group: Stanford BIOE44 - S11   (2012-12-07)
Revision as of 10:33, 7 December 2012 by Registry (Talk | contribs)

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K12 e.coli native Manganese Superoxide Dismutase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 346
    Illegal XhoI site found at 43
    Illegal XhoI site found at 127
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
    Illegal NgoMIV site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico.


Source

This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.

References