Part:BBa_K801020:Design

KlADH4 yeast promoter, ethanol inducible

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 753
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 590
  

To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis.

The feature annotation in the UASe region (Upstream activating sequence "ethanol") is taken from:

• Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. PMID: 10724480

Extracted from genomic DNA of K. lactis using PCR.

Keywords: ethanol inducible, KlADH4, yeast, promoter, inducible, K. lactis, S. cerevisiae
Abbreviations:

Design Notes

Related BioBrick:

• Other versions:BBa_K678001: PalcA, inducible Aspergillus nidulans promoter
• Part was totally sequenced

Backbone:

• Backbone name: pSB1C3'
• Resistance: Cam
• Copynumber: high

Protein coding:
-

Enzymatic activity: -

Cytotoxicity:

• none

Safety notes:

• Known and anticipated sefety issues: none
• Known and anticipated security issues: none

Intellectual property:

Corresponding part author/authors:

Source

Source:

• Cloned from genomic DNA of Kluyveromyces lactis strain WSYC115
• Forward Primer:
  5'- AGT CAG TCT AGA ACG ACG GTA CAT AAG AAT GAG G - 3'
  

Organism:

• Genesequence derived from Kluyveromyces lactis

References

Literature references:

Database references: