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Revision as of 02:32, 4 October 2012 by Lisa.O (Talk | contribs) (→‎References)

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Beta-D-glucoside glucohydrolase (bglX) with strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1639
    Illegal AgeI site found at 1861
    Illegal AgeI site found at 2050
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An illegal pstI site was removed from the 15th and 16th amino acid positions via site directed mutagenesis.


Source

The beta-D-glucoside glucohydrolase (bglX) gene was amplified from TOP10 E. coli via polymerase chain reaction.

References

Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S.The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65.