Coding

Part:BBa_K726018

Designed by: Veronica Zepeda   Group: iGEM12_UCSF   (2012-10-02)
Revision as of 20:52, 3 October 2012 by Vzepeda (Talk | contribs)

T7Prom+HisTag+VioDC

This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).


Usage and Biology

A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment. However, when cells containing this construct were grown together with strains containing the above construct (VioABE), a purple pigment (violacein) was produced. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2571
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1063
    Illegal SapI.rc site found at 1138


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Categories
Parameters
None