Device
tuned pArs

Part:BBa_K935002:Design

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)
Revision as of 04:27, 3 October 2012 by Geotwaddle (Talk | contribs) (→‎Source)

E. coli ArsR under the control of pArs coupled with pArs + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1316
    Illegal AgeI site found at 1428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Linked generators, one of ArsR under pArs and a separate RFP under pArs. The basal transcription rate of ArsR was found to raise the threshold of the production of RFP, essentially setting or "tuning" the latter generator. See related parts BBa_K935004 and BBa_K935005.

Source

BBa_J33201 and BBa_K190015 with some modifications

References