Composite
Part:BBa_K917005
Designed by: Elitsa Peeva Group: iGEM12_Edinburgh (2012-09-20)
lac promoter plus nitroreductase (nsfI) gene from Enterobacter cloacae
This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductaseunder the control of the lac promoter. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of metronidazole leads to growth inhibition (see Experience page). This gene could possibly could be counter selectable marker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1189
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |