Part:BBa_K590025:Experience

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••••• 2012 UIUC iGEM

As a side project, we decided to characterize the University of Washington's existing biobrick, the Petrobrick. After analyzing the results from their experiment, we decided to reproduce the results specifically for the detection of C15 alkanes, as they were the most abundant. Transformed cells were grown in TB media and then subsequently in M9 min-glucose media to produce the alkanes. Using Gas Chromotography-Mass Spectometry, a standard curve was created using the known concentrations of four control samples of C15 alkanes and their corresponding peak areas. Then, GCMS analysis of four samples was used to find the concentration yields based on the standard curve. The average retention time for C15 alkanes was determined to be at 11.47 s, according to the chromatogram analysis. Our data was in sync with the University of Washington results, as their average yield of C15 alkanes was 160.3 mg/L and our range was from 130 to 190 mg/L. Based on our results, we were able to successfully reproduce the Petrobrick characterization.

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