Coding
TapZ

Part:BBa_K845005

Designed by: Grégory Hansen   Group: iGEM12_Grenoble   (2012-09-26)
Revision as of 17:00, 30 September 2012 by Greghansen (Talk | contribs)

TapZ engineered membrane receptor

In order to detect the S.Aureus we take inspiration on the parasite project ( http://2010.igem.org/Team:Imperial_College_London ) but there was one problem there is no peptide detector in E.coli who was able to activate a promoter. One receptor, involved in chemotaxis, detects few di/tri peptides (Tap receptor). In order to activate a promoter in presence of that peptide we designed a hybrid Tap/EnvZ receptor (which we called TapZ). EnvZ is a receptor involved in osmotic pressure sensing. It phosphorylates ompR which activates the pompC promoter. We must use this part in a BW25113 ΔenvZ.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/receptor
Parameters
None