Composite
pBAD-GFP

Part:BBa_K845000

Designed by: Grégory Hansen   Group: iGEM12_Grenoble   (2012-09-26)
Revision as of 16:42, 29 September 2012 by Greghansen (Talk | contribs) (→‎Functional Parameters)

iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).

In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.

The activity of the promoter was tested under several conditions ( http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test ) using a GFP as a reporter (on a pUA66). We tested this activity in BW25113 ΔcyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 500
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 344
    Illegal XhoI site found at 494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 500
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 500
    Illegal AgeI site found at 179
    Illegal AgeI site found at 484
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1169
    Illegal SapI site found at 161


Functional Parameters

BBa_K845001 parameters: cAMP-CRP complex Not understood

[edit]
Categories
//function/reporter/fluorescence
//promoter
Parameters
emission504 nm
excitation395 and 475 nm
negative_regulatorsAraC protein
positive_regulatorscAMP-CRP complex and Arabinose