Generator

Part:BBa_K819008:Design

Designed by: ZHANG Zidong   Group: iGEM12_Peking   (2012-09-03)
Revision as of 19:06, 26 September 2012 by Zhangzidong (Talk | contribs) (→‎Design Notes)

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Lux Operon under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726


Design Notes

Lux CDABEG genes and related RBS from luxbrick(BBa_K325909) are placed under T7 promoter (BBa_I712074).

Source

Lux brick (BBa_K325909) and T7 promoter (BBa_I712074) are from 2012 spring distribution kit.

References

1. Slock, J.(1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri. Am.Biology.Teacher, 57:225:227
2. Meighen, E.A.(1994) Genetics of bacterial bioluminescence. Annu.Rev.Genet., 28:117:139