Coding
Ruxyn

Part:BBa_K805012

Designed by: Liu Ping   Group: iGEM12_HUST-China   (2012-09-20)
Revision as of 11:56, 26 September 2012 by HUST XueYu (Talk | contribs)

Ruxyn1 Glycosyl hydrolase (GH) family 43 b-Dxylosidase/a-L-arabinofuranosidase Ruxyn can hydrolyze p-nitrophenyl-b-D-xylopyranoside (pNPX), p-nitrophenyla-L-arabinofuranoside (pNPA), and xylo-oligosaccharide substrates. Ruxyn shows high synergism with endoxylanase,greatly elevating the reducing sugars released from brichwood xylans, and converted most intermediate xylo-oligosaccharide hydrolysate into xylose.


We construct the gene on the carrier displaying on the surface of Pichia pastoris and express it it in Pichia pastoris with electroporation. Then we detect the expression on the surface of Pichia pastoris by observing with fluorescent microscope and by flow cytometry analysis.

Luorescent microscope 5.jpg Luorescent microscope 6.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 373
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None