Coding
Cel5A

Part:BBa_K805011:Design

Designed by: Liu Ping   Group: iGEM12_HUST-China   (2012-09-20)
Revision as of 11:40, 26 September 2012 by HUST XueYu (Talk | contribs) (→‎Source)

Cel5A, endoglucanases


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 763
    Illegal AgeI site found at 1027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024


Design Notes

sequence confirmed

Source

Extracted from host genome Penicillium decumbens 114-2

References

Qu W, Shao W,Cloning, expression and characterization of glycoside hydrolase family 11 endoxylanase from Bacillus pumilus ARA.Biotechnol Lett. 2011 Jul;33(7):1407-16. Epub 2011 Mar 3.