Coding
SYFP2

Part:BBa_K864100:Design

Designed by: Erik Gullberg   Group: iGEM12_Uppsala_University   (2012-09-24)
Revision as of 23:11, 24 September 2012 by Erigu863 (Talk | contribs) (→‎References)

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Super Yellow Fluorescent Protein 2 (SYFP2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been codon optimized for expression in E coli by DNA 2.0.


Source

Amino acid sequence taken from the article by Kremers et al 2006.

References

[http://pubs.acs.org/doi/abs/10.1021/bi0516273] Kremers, G.-J., Goedhart, J., van Munster, E. B., & Gadella, T. W. J. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570–6580. doi:10.1021/bi0516273