Composite

Part:BBa_K917005

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-20)
Revision as of 13:33, 20 September 2012 by Registry (Talk | contribs)

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lac promoter plus nitroreductase (nsfI) gene from Enterobacter cloacae

This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductase under the control of the lac promoter.Expression of this gene in the presence of metronidazole leads to growth inhibition. This gene could possibly could be counter selectable marker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1189
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None