Coding

Part:BBa_K777100:Design

Designed by: Team Goettingen   Group: iGEM12_Goettingen   (2012-09-15)
Revision as of 11:40, 15 September 2012 by NikoGoe (Talk | contribs) (→‎Design Notes)

flhDC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The PstI restriction site was mutated to CTGCGG using the reverse primer.
  • flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: ACTGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTG
    • Rev: TCCTGCAGCGGCCGCTACTAGTTACTGCCCGGGATGGCGGTTGACATAAGCcGCAGGCAAAGCTGCCAACAG
      (the uncapitalized "c" induces the mutation for removal of the PstI site)

Source

The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

References