Part:BBa_K731710
Platform for terminators analysis under the control of tac promoter
This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus)allow to obtain several information about terminator's presence effect. The vector was obained by mutagenesis of BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (lacO). The combined use of BBa_K731710 and [[1]] allow also to analyse any potential difference in terminators' activity due to different polymerases.
The two fluorescent proteins have partially overlapping emission spectra, thus, to avoid unwanted interferences, the fluorimetric measures should be taken with these parameters:
Venus excitation: 485nm, Venus emission: 528nm
Cherry excitation: 528nm, Cherry emission: 615nm.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6865
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 6871 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6865
Illegal BglII site found at 6011
Illegal BamHI site found at 6847
Illegal XhoI site found at 753 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 6865
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 6865
Plasmid lacks a suffix.
Illegal XbaI site found at 6880
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 714
Illegal NgoMIV site found at 1051
Illegal NgoMIV site found at 4231
Illegal NgoMIV site found at 4391
Illegal NgoMIV site found at 5979
Illegal AgeI site found at 6815 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2228
Illegal SapI.rc site found at 3310
| None |