Part:BBa_K584020

AFP extracellular protein generator

This part contains BBa_K584019 [ OmpA_transmembrane_domain + Linker + point_mutant_HPLC6(AFP) ] with the B0034 RBS, B0015 Terminator.

A point mutation at Base Pair number 156 of the HPLC6 sequence was performed by replacing the T in the wild-type coding sequence to C. This mutation was necessary as the sequence CTGCAG in the Wild-type coding sequence corresponded to a Pst1 restriction site. GCT(Wild type) and GCC(1 point mutant) are both valid triplet codons for Alanine.

Detailed information of the Pseudopleuronectes americanus Antifreeze Protein (HPLC6 gene) can be found on this NCBI [http://www.ncbi.nlm.nih.gov/nuccore/M62417 webpage].

This brick was synthesized and sequenced by [http://www.eurogentec.com/countryselector.html Eurogentec]. Its sequence is correct.

After the regional jamboree we characterized the antifreeze protein [http://2011.igem.org/Team:KULeuven/Results qualitatively] and [http://2011.igem.org/Team:KULeuven/Results quantitatively].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]