Regulatory
Part:BBa_K656010
Designed by: Evan Clark, Julius Ho, Eli Moss, Jesse Palmer Group: iGEM11_Brown-Stanford (2011-09-19)
Anabaena pSac Promoter
Anabaena vegetative cell promoter, linked to photosystem I. Can be used to express specifically in vegetative cells, excluding heterocyst expression.
This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |