Part:BBa_K633003

Extracellular Succrase Sacc

Sacc Extracellular Sucrase

This extracellular sucrase is an enzyme from Zymomonas mobilis. The expression product is different from SacB; showing sucrase but not levansucrase activity. In E. coli BL21, after induction of the T7 polymerase expression system with IPTG. Subcellular fractionation of the E. coli clone carrying plasmid pLSS2811 showed that more than 70% of the sucrase activity could be detected in the cytoplasmic fraction, suggesting that the enzyme was soluble and not secreted in E. coli. Its structure is monomeric, with an optimum temperature 20 to 40 ° C, pH of 2.5-7.5 and a size of 48 kDa. It also has a free N-terminus.
This part was cloned from Zymmomonas mobilis in the FEMSA Biotechnology Center by phD. Jose Manuel Aguilar, in team TEC-Monterrey's campus.
The cloning technique was the next:

Kannan R, Mukundan G, Aït-Abdelkader N, Augier-Magro V, Baratti J, Gunasekaran P. Molecular cloning and characterization of the extracellular sucrase gene (sacC) of Zymomonas mobilis. Arch Microbiol. 1995 Mar;163(3):195-204.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 907
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 607