DNA

Part:BBa_K676012:Design

Designed by: Meng Li   Group: iGEM11_UCL_London   (2011-09-19)
Revision as of 02:19, 10 October 2011 by Eintisar (Talk | contribs) (→‎Source)

Gyrase Binding Site from pBR322


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Performed PCR to clone out the GBS from the pBR322 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.

Source

pBR322 Plasmid DNA

References