Reporter

Part:BBa_K598001:Design

Designed by: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 19:22, 5 October 2011 by Hsy (Talk | contribs)

Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 730


Design Notes

The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well. Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.


Source

Use mutagenesis PCR of BBa_K598007 to alter RBS sequence.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004) A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research, 32, 1610-1614.