Generator

Part:BBa_K581009:Design

Designed by: Handuo SHI, Dingqiao Wen   Group: iGEM11_Peking_S   (2011-09-26)
Revision as of 18:40, 5 October 2011 by Nuanuan128 (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

pqrr-GFP ssrA degradation tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 936
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 831


Design Notes

In the existence of luxU, CqsS and luxO, the part serves as an inverter: it would generate GFP without the inducement of CAI-1, while CAI-1 would shut it down.


Source

The promoter qrr is from Vibrio cholera.

References