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Generator

Part:BBa_K581011

Designed by: Handuo SHI, Dingqiao Wen   Group: iGEM11_Peking_S   (2011-09-26)
Revision as of 15:47, 5 October 2011 by Nuanuan128 (Talk | contribs)

pBAD-rbs-cqsA-terminator

A generator of CAI-1 behind pBAD promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

▪ A silent mutation (G->A) at base 1116 was made to remove XbaI site

▪ cqsA was amplified by PCR


Source

▪ We received the genome DNA of Vibrio cholera from Chinese Center for Disease Control and Prevention

▪ Although our system includes several genes from Vibrio cholera, these genes are not directly involved with virulence or antibiotic resistance in any case.

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Parameters
None