Regulatory

Part:BBa_J23103:Experience

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-04)
Revision as of 14:15, 5 October 2011 by GeorgeLeung (Talk | contribs)

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Applications of BBa_J23103

User Reviews

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iGEM HKU 2011

To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain. The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression.

mRFP fluorescence intensity under different promoters

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