Measurement

Part:BBa_K518013:Design

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-10-03)
Revision as of 09:59, 5 October 2011 by Mogi (Talk | contribs)

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sulA promoter evaluation device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1850
    Illegal NheI site found at 1873
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2452
    Illegal SapI.rc site found at 887


Design Notes

sulAp + dual luciferase reporter construct

When you are going to evaluate SOS promoter, you must use recA(+) strain.


Source

sulAp (BBa_K518010) was cloned from Escherichia coli K12 strain's genome.

References