Device

Part:BBa_K598010

Designed by: Siyang HAO, Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 08:49, 5 October 2011 by Hsy (Talk | contribs)

pBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015

This is a measurement with a theophylline responsive riboswitch and reporter gene, GFP. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP measured by GFP fluorescence intensity. In this part, the theophylline responsive riboswitch contains an engineered ribosome binding site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 868


For the full characterisation of the device, please refer to [http://https://parts.igem.org/wiki/index.php?title=Part:BBa_K598006 BBa_K598006]

[edit]
Categories
Parameters
chassisE. coli DH5α
ligandstheophylline
n/apBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015
rbsrobust
resistanceChloramphenicol