Regulatory

Part:BBa_K649000

Designed by: Tomoyuki Ohno   Group: iGEM11_Tokyo_Tech   (2011-09-26)
Revision as of 00:57, 5 October 2011 by Takuya 1613 (Talk | contribs)

LasR and 3OC12HSL regulated promoter.

In the presence of 3OC12HSL lasR can bind to the operator of this promoter and activate the transcription of downstream gene, whereas in the absence of 3OC12HSL lasR can't bind to the operator and can't activate the transcription.


To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. If you want to see detailed infomation, please click here.


We improved previous lasI promoter(BBa_J64010).our assay of BBa_J64010


For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#3. our work in Tokyo_Tech 2011 wiki]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aLasR and 3OC12HSL regulated promoter.