Composite

Part:BBa_K646003

Designed by: David Dubow   Group: iGEM11_Macquarie_Australia   (2011-09-29)
Revision as of 08:30, 4 October 2011 by Eddymoh (Talk | contribs)

T7 promoter with Hemeoxygenase

Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI.

Successful expression should result in green colonies due to the presence of biliverdin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None