Generator

Part:BBa_K518004:Experience

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-09-15)
Revision as of 19:13, 3 October 2011 by Mogi (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K518004

User Reviews

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UT-Tokyo 2011

We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (Chao, Lo, & Luo, 2000), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin reaction and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our result page].

Reference

Chao, Y., Lo, T., & Luo, N. (2000). Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli. Enzyme Microb Technol, 27(1-2), 19-25.


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