Coding

Part:BBa_K518003

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-09-15)
Revision as of 14:56, 3 October 2011 by Mogi (Talk | contribs)

LexA - a repressor for LexA-regulated SOS promoters -

Escherichia coli and other organisms have a gene cluster activated in case of emergency, which is called "SOS genes". It is well-documented that in E. coli, most of those SOS genes are repressed by LexA protein. When the cell is exposed to excess stress, RecA is up-produced, which promotes the self-degradation of LexA. As a result, many LexA-regulated genes become expressed. Combined with RecA and SOS promoters (for instance, sulAp), a stress-responsive gene-expression circuit was assembled. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 684


[edit]
Categories
//cds/transcriptionalregulator/repressor
Parameters
None