RNA

Part:BBa_K598005:Design

Designed by: Siyang HAO   Group: iGEM11_Peking_R   (2011-08-15)
Revision as of 05:57, 3 October 2011 by Hsy (Talk | contribs) (→‎Source)

Theophylline Responsive Riboswitch P1G1 with Native RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Obtain sequence of this RNA controller from original paper. Primer design. Insert RNA controller sequence into backbone via PCR and bluntend ligation.

Source

Sequence is based on original design of Beatrix Suess. Insert RNA controller sequence into backbone pSB1C3 via PCR and bluntend ligation.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.