Part:BBa_R0079:Experience

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User Reviews

UNIQ2c0654c84347a0e3-partinfo-00000000-QINU UNIQ2c0654c84347a0e3-partinfo-00000001-QINU

No review score entered. NYMU-Taipei 2009

We have characterised the strength of the promoters pCI, p22, pLux, pLas relative to pCI. More details are available at the [http://2009.igem.org/Team:NYMU-Taipei/Project/Promoter_Strength_Testing NYMU-Taipei iGEM09 wiki].

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No review score entered. Northwestern 2011

The Northwestern iGEM team used this part as a unit within our Pseudomonas Aeruginosa biosensor. When this LasR/PAI regulated promoter is induced at varying concentrations of PAI in the presence of excess LasR, we observed GFP fluorescence in accordance with the graph below.

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••• Tokyo-tech iGEm 2011

Judging from Northwestern iGEM 2011 team's data, in the presence of 3O-C12-HSL, the culture showed about 3-fold higher fluorescence intensity than that in the absence of 3O-C12-HSL. We improved this part. LasI promoter(BBa_K649000) which we constructed was more successfully regulated by 3O-C12-HSL.

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