Device

Part:BBa_K533005:Design

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 14:35, 29 September 2011 by Registry (Talk | contribs)

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OmpA-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His tag is inserted between OmpA and GFP for easier detection in Western Blotting.


Source

OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1

References